Functionalization of colloidal nanoparticles with a discrete number of ligands based on a "HALO-bioclick" reaction.
Chem Commun (Camb)
; 56(77): 11398-11401, 2020 Sep 29.
Article
en En
| MEDLINE
| ID: mdl-32990290
ABSTRACT
A recombinant HALO-GFP fusion protein was designed and isolated to demonstrate the feasibility of controlling the number and orientation of protein ligands to be conjugated on colloidal gold nanoparticles. AuNPs functionalized with exactly one or exactly two GFP molecules exhibited fully preserved functionality of the protein. The method is very straightforward and generally provides highly bioactive nanoparticle-protein conjugates.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Fluorescentes Verdes
/
Nanopartículas del Metal
/
Oro
Idioma:
En
Revista:
Chem Commun (Camb)
Asunto de la revista:
QUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Italia