Rapid nucleotide sequence analysis of the small subunit ribosomal RNA of Toxoplasma gondii: evolutionary implications for the Apicomplexa.
Mol Biochem Parasitol
; 25(3): 239-46, 1987 Oct.
Article
en En
| MEDLINE
| ID: mdl-3320746
ABSTRACT
A method for obtaining a large proportion of the nucleotide sequence of the small subunit ribosomal RNA (srRNA) was applied to the obligate intracellular protozoon Toxoplasma gondii. The method uses reverse transcription of as little as 8 micrograms of total cellular RNA. This fast, efficient method has numerous advantages over traditional gene cloning methods when nucleotide sequences are required for evolutionary studies. A phylogenetic analysis of the srRNA sequence data showed that T. gondii is not especially closely related to any other organism for which srRNA sequences are available, including another member of the Apicomplexa.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Toxoplasma
/
ARN Ribosómico
Límite:
Animals
Idioma:
En
Revista:
Mol Biochem Parasitol
Año:
1987
Tipo del documento:
Article