Hydrophilic carrageenan functionalized magnetic carbon-based framework linked by silane coupling agent for the enrichment of N-glycopeptides from human saliva.
J Sep Sci
; 44(10): 2143-2152, 2021 May.
Article
en En
| MEDLINE
| ID: mdl-33734567
ABSTRACT
In this work, a magnetic graphene material coated with mesoporous silica was selected as the substrate, 3-glycidoxypropyltrimethoxysilane and polyethyleneimine were sequentially bonded through chemical reactions, and then carrageenan was successfully introduced by electrostatic interaction; finally, hydrophilic nanocomposite material was prepared. Due to the large number of hydrophilic groups, and polyethyleneimine was connected by means of chemical bonds, this material exhibits good hydrophilicity and stability for glycopeptide enrichment. In the actual enrichment process, nanomaterial exhibits high selectivity (1500), high sensitivity (2 fmol), and good repeatability (five cycles). In addition, the synthesized material also shows a good enrichment effect in the face of actual complex biological samples, which captured 40 N-glycopeptides from human saliva, indicating the application potential for enrichment of N-glycopeptides.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polietileneimina
/
Saliva
/
Silanos
/
Carbono
/
Glicopéptidos
/
Carragenina
/
Extracción en Fase Sólida
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Sep Sci
Año:
2021
Tipo del documento:
Article