Noninvasive Laser Microtomography of a Human Isolated Oocyte.

Pogorelov, A G; Makarova, N P; Sysoeva, A P; Pogorelova, V N; Sukhikh, G T

Pogorelov, A G; Makarova, N P; Sysoeva, A P; Pogorelova, V N; Sukhikh, G T.

Afiliación

Pogorelov AG; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia. agpogorelov@rambler.ru.
Makarova NP; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Sysoeva AP; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
Pogorelova VN; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, Russia.
Sukhikh GT; V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.

Bull Exp Biol Med ; 171(1): 32-36, 2021 May.

Article en En | MEDLINE | ID: mdl-34046786

RESUMEN

Using a non-invasive approach, quantitative laser scanning microtomography (QLSM), the morphology of human oocyte was studied layer-by-layer. Then, the cell volume was computed based on 3D reconstruction of a stack of optical sections obtained by QLSM. The integrity of oocyte membrane after cryopreservation was assessed by measuring the changes in oocyte volume in response to hypotonic shock.

Asunto(s)

Criopreservación; Oocitos; Tamaño de la Célula; Criopreservación/métodos; Humanos; Rayos Láser; Luz; Oocitos/metabolismo

Palabras clave

cryopreservation; hypotonic shock; mature oocyte of human and mice; non-invasive microtomography; quantitative 3D reconstruction

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Oocitos / Criopreservación Límite: Humans Idioma: En Revista: Bull Exp Biol Med Año: 2021 Tipo del documento: Article País de afiliación: Rusia

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