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Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.
Hasan, Mohammad R; Kalikiri, Mahesh K R; Mirza, Faheem; Sundararaju, Sathyavathi; Sharma, Anju; Xaba, Thabisile; Lorenz, Stephan; Chemaitelly, Hiam; El-Kahlout, Reham A; Tsui, Kin Ming; Yassine, Hadi M; Coyle, Peter V; Al Khal, Abdullatif; Bertollini, Roberto; Al Thani, Mohamed H; Abu-Raddad, Laith J; Tang, Patrick.
Afiliación
  • Hasan MR; Department of Pathology, Sidra Medicine, Doha, Qatar; Pathology and Laboratory Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar. Electronic address: mhasan@sidra.org.
  • Kalikiri MKR; Department of Research, Sidra Medicine, Doha, Qatar.
  • Mirza F; Department of Pathology, Sidra Medicine, Doha, Qatar.
  • Sundararaju S; Department of Pathology, Sidra Medicine, Doha, Qatar.
  • Sharma A; Department of Pathology, Sidra Medicine, Doha, Qatar.
  • Xaba T; Department of Pathology, Sidra Medicine, Doha, Qatar.
  • Lorenz S; Department of Research, Sidra Medicine, Doha, Qatar.
  • Chemaitelly H; Infectious Disease Epidemiology Group, Doha, Qatar; World Health Organization Collaborating Centre for Disease Epidemiology Analytics on HIV/AIDS, Sexually Transmitted Infections, and Viral Hepatitis, Doha, Qatar.
  • El-Kahlout RA; Hamad Medical Corporation, Doha, Qatar.
  • Tsui KM; Department of Pathology, Sidra Medicine, Doha, Qatar; Pathology and Laboratory Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar.
  • Yassine HM; Biomedical Research Center, Qatar University, Doha, Qatar.
  • Coyle PV; Hamad Medical Corporation, Doha, Qatar; Biomedical Research Center, Qatar University, Doha, Qatar; Welcome-Wolfson Institute for Experimental Medicine, Queens University, Belfast, UK.
  • Al Khal A; Hamad Medical Corporation, Doha, Qatar.
  • Bertollini R; Ministry of Public Health, Doha, Qatar.
  • Al Thani MH; Ministry of Public Health, Doha, Qatar.
  • Abu-Raddad LJ; Infectious Disease Epidemiology Group, Doha, Qatar; World Health Organization Collaborating Centre for Disease Epidemiology Analytics on HIV/AIDS, Sexually Transmitted Infections, and Viral Hepatitis, Doha, Qatar.
  • Tang P; Department of Pathology, Sidra Medicine, Doha, Qatar; Pathology and Laboratory Medicine, Weill Cornell Medicine-Qatar, Doha, Qatar.
Int J Infect Dis ; 112: 52-54, 2021 Nov.
Article en En | MEDLINE | ID: mdl-34525398
ABSTRACT
Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Screening_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Int J Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2021 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: SARS-CoV-2 / COVID-19 Tipo de estudio: Screening_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Int J Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2021 Tipo del documento: Article