Amoebicidal Activity of Poly-Epsilon-Lysine Functionalized Hydrogels.
Invest Ophthalmol Vis Sci
; 63(1): 11, 2022 01 03.
Article
en En
| MEDLINE
| ID: mdl-34994769
ABSTRACT
Purpose:
To determine the amoebicidal activity of functionalized poly-epsilon-lysine hydrogels (pÉK+) against Acanthamoeba castellanii.Methods:
A. castellanii trophozoites and cysts were grown in the presence of pÉK solution (0-2.17 mM), pÉK or pÉK+ hydrogels, or commercial hydrogel contact lens (CL) for 24 hours or 7 days in PBS or Peptone-Yeast-Glucose (PYG) media (nutrient-deplete or nutrient-replete cultures, respectively). Toxicity was determined using propidium iodide and imaged using fluorescence microscopy. Ex vivo porcine corneas were inoculated with A. castellanii trophozoites ± pÉK, pÉK+ hydrogels or commercial hydrogel CL for 7 days. Corneal infection was assessed by periodic acid-Schiff staining and histologic analysis. Regrowth of A. castellanii from hydrogel lenses and corneal discs at 7 days was assessed using microscopy and enumeration.Results:
The toxicity of pÉK+ hydrogels resulted in the death of 98.52% or 83.31% of the trophozoites at 24 hours or 7 days, respectively. The toxicity of pÉK+ hydrogels resulted in the death of 70.59% or 82.32% of the cysts in PBS at 24 hours or 7 days, respectively. Cysts exposed to pÉK+ hydrogels in PYG medium resulted in 75.37% and 87.14% death at 24 hours and 7 days. Ex vivo corneas infected with trophozoites and incubated with pÉK+ hydrogels showed the absence of A. castellanii in the stroma, with no regrowth from corneas or pÉK+ hydrogel, compared with infected-only corneas and those incubated in presence of commercial hydrogel CL.Conclusions:
pÉK+ hydrogels demonstrated pronounced amoebicidal and cysticidal activity against A. castellanii. pÉK+ hydrogels have the potential for use as CLs that could minimize the risk of CL-associated Acanthamoeba keratitis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polilisina
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Queratitis por Acanthamoeba
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Infecciones Parasitarias del Ojo
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Córnea
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Hidrogeles
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Acanthamoeba castellanii
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Amebicidas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Año:
2022
Tipo del documento:
Article
País de afiliación:
Reino Unido