Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus.
Arch Virol
; 168(3): 86, 2023 Feb 11.
Article
en En
| MEDLINE
| ID: mdl-36773166
ABSTRACT
A new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5' and 3' untranslated regions (UTRs), followed by a poly(A) tail at the 3' end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organization and phylogenetic relationship to other nepoviruses suggested that this virus is a novel member of subgroup B, and recombination analysis revealed its evolutionary history within the subgroup. These results suggest the new virus, provisionally named "horse nettle virus A", represents a new species within the genus Nepovirus.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nepovirus
/
Solanum
Idioma:
En
Revista:
Arch Virol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos