Knockdown of LRCH4 Remodels Tumor Microenvironment Through Inhibiting YAP and TGF-ß/Smad Signaling Pathway in Colorectal Cancer.
Comb Chem High Throughput Screen
; 27(12): 1823-1829, 2024.
Article
en En
| MEDLINE
| ID: mdl-38383956
ABSTRACT
BACKGROUND:
Colorectal cancer is one of the most common gastrointestinal malignancies worldwide. LRCH4 is the top 1 gene associated with an unfavorable prognosis in colorectal cancer.METHODS:
Here, we reported that the knockdown of LRCH4 inhibited the proliferation, migration and invasion in HT29 cells.RESULTS:
The activity of Yes-Associated Protein (YAP), a transcription factor in the Hppo-YAP signaling pathway, was significantly inhibited by LRCH4-siRNA. LRCH4 knockdown also reversed the EMT and regulated the expression of extracellular matrix (ECM) protein, Fibronectin and Collagen IV in HT29 cells. In addition, the TGF-ß/Smad signaling pathway, as the downstream pathway of Yap, was also inhibited by LRCH4 knockdown.CONCLUSION:
Knockdown of LRCH4 involved in the regulation of ECM and EMT and inhibited YAP and the TGF-ß/Smad signaling pathway in colorectal cancer cells. Our study provided a mechanism of LRCH4 on colorectal cancer cells, and a new potential target for clinical tumor treatment.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Neoplasias Colorrectales
/
Transducción de Señal
/
Factor de Crecimiento Transformador beta
/
Proliferación Celular
/
Proteínas Smad
/
Microambiente Tumoral
Límite:
Humans
Idioma:
En
Revista:
Comb Chem High Throughput Screen
Asunto de la revista:
BIOLOGIA MOLECULAR
/
QUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China