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The stability of 65 biochemistry analytes in plasma, serum, and whole blood.
Zhou, Janet; Fabros, Anselmo; Lam, Sarah Jane; Coro, Anna; Selvaratnam, Rajeevan; Brinc, Davor; Di Meo, Ashley.
Afiliación
  • Zhou J; Department of Laboratory Medicine & Pathobiology, 233837 University of Toronto , Toronto, ON, Canada.
  • Fabros A; Department of Clinical Biochemistry, 574811 University Health Network , Toronto, ON, Canada.
  • Lam SJ; Department of Clinical Biochemistry, 574811 University Health Network , Toronto, ON, Canada.
  • Coro A; Department of Clinical Biochemistry, 574811 University Health Network , Toronto, ON, Canada.
  • Selvaratnam R; Department of Laboratory Medicine & Pathobiology, 233837 University of Toronto , Toronto, ON, Canada.
  • Brinc D; Department of Clinical Biochemistry, 574811 University Health Network , Toronto, ON, Canada.
  • Di Meo A; Department of Laboratory Medicine & Pathobiology, 233837 University of Toronto , Toronto, ON, Canada.
Clin Chem Lab Med ; 62(8): 1557-1569, 2024 Jul 26.
Article en En | MEDLINE | ID: mdl-38443327
ABSTRACT

OBJECTIVES:

The pre-analytical stability of various biochemical analytes requires careful consideration, as it can lead to the release of erroneous laboratory results. There is currently significant variability in the literature regarding the pre-analytical stability of various analytes. The aim of this study was to determine the pre-analytical stability of 65 analytes in whole blood, serum and plasma using a standardized approach.

METHODS:

Blood samples were collected from 30 healthy volunteers (10 volunteers per analyte) into five vacutainers; either SST, Li-heparin, K2-EDTA, or Na-fluoride/K-oxalate. Several conditions were tested, including delayed centrifugation with storage of whole blood at room temperature (RT) for 8 h, delayed centrifugation with storage of whole blood at RT or 4 °C for 24 h, and immediate centrifugation with storage of plasma or serum at RT for 24 h. Percent deviation (% PD) from baseline was calculated for each analyte and compared to the maximum permissible instability (MPI) derived from intra- and inter-individual biological variation.

RESULTS:

The majority of the analytes evaluated remained stable across all vacutainer types, temperatures, and timepoints tested. Glucose, potassium, and aspartate aminotransferase, among others, were significantly impacted by delayed centrifugation, having been found to be unstable in whole blood specimens stored at room temperature for 8 h.

CONCLUSIONS:

The data presented provides insight into the pre-analytical variables that impact the stability of routine biochemical analytes. This study may help to reduce the frequency of erroneous laboratory results released due to exceeded stability and reduce unnecessary repeat phlebotomy for analytes that remain stable despite delayed processing.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plasma / Recolección de Muestras de Sangre / Suero Límite: Adult / Female / Humans / Male Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plasma / Recolección de Muestras de Sangre / Suero Límite: Adult / Female / Humans / Male Idioma: En Revista: Clin Chem Lab Med Asunto de la revista: QUIMICA CLINICA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Canadá