Top-down mass spectrometry of native proteoforms and their complexes: a community study.

Habeck, Tanja; Brown, Kyle A; Des Soye, Benjamin; Lantz, Carter; Zhou, Mowei; Alam, Novera; Hossain, Md Amin; Jung, Wonhyeuk; Keener, James E; Volny, Michael; Wilson, Jesse W; Ying, Yujia; Agar, Jeffrey N; Danis, Paul O; Ge, Ying; Kelleher, Neil L; Li, Huilin; Loo, Joseph A; Marty, Michael T; Pasa-Tolic, Ljiljana; Sandoval, Wendy; Lermyte, Frederik

Habeck, Tanja; Brown, Kyle A; Des Soye, Benjamin; Lantz, Carter; Zhou, Mowei; Alam, Novera; Hossain, Md Amin; Jung, Wonhyeuk; Keener, James E; Volny, Michael; Wilson, Jesse W; Ying, Yujia; Agar, Jeffrey N; Danis, Paul O; Ge, Ying; Kelleher, Neil L; Li, Huilin; Loo, Joseph A; Marty, Michael T; Pasa-Tolic, Ljiljana; Sandoval, Wendy; Lermyte, Frederik.

Afiliación

Habeck T; Technische Universität Darmstadt, Darmstadt, Germany.
Brown KA; University of Wisconsin-Madison, Madison, WI, USA.
Des Soye B; Northwestern University, Evanston, IL, USA.
Lantz C; University of California, Los Angeles, CA, USA.
Zhou M; Pacific Northwest National Laboratory, Richland, WA, USA.
Alam N; Zhejiang University, Zhejiang, China.
Hossain MA; Northeastern University, Boston, MA, USA.
Jung W; Northeastern University, Boston, MA, USA.
Keener JE; University of California, Los Angeles, CA, USA.
Volny M; University of Arizona, Tuscon, AZ, USA.
Wilson JW; Genentech Inc., San Francisco, CA, USA.
Ying Y; Pacific Northwest National Laboratory, Richland, WA, USA.
Agar JN; Sun Yat-sen University, Guangzhou, China.
Danis PO; Northeastern University, Boston, MA, USA.
Ge Y; Consortium for Top-Down Proteomics, Cambridge, MA, USA.
Kelleher NL; Consortium for Top-Down Proteomics, Cambridge, MA, USA.
Li H; University of Wisconsin-Madison, Madison, WI, USA.
Loo JA; Consortium for Top-Down Proteomics, Cambridge, MA, USA.
Marty MT; Northwestern University, Evanston, IL, USA.
Pasa-Tolic L; Consortium for Top-Down Proteomics, Cambridge, MA, USA.
Sandoval W; Sun Yat-sen University, Guangzhou, China.
Lermyte F; University of California, Los Angeles, CA, USA.

Nat Methods ; 2024 May 14.

Article en En | MEDLINE | ID: mdl-38744918

ABSTRACT

ABSTRACT

The combination of native electrospray ionization with top-down fragmentation in mass spectrometry (MS) allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and cofactors. Although this approach is powerful, both native MS and top-down MS are not yet well standardized, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics initiated a study to develop and test protocols for native MS combined with top-down fragmentation of proteins and protein complexes across 11 instruments in nine laboratories. Here we report the summary of the outcomes to provide robust benchmarks and a valuable entry point for the scientific community.

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2024 Tipo del documento: Article País de afiliación: Alemania

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