Your browser doesn't support javascript.
loading
Space radiation damage rescued by inhibition of key spaceflight associated miRNAs.
McDonald, J Tyson; Kim, JangKeun; Farmerie, Lily; Johnson, Meghan L; Trovao, Nidia S; Arif, Shehbeel; Siew, Keith; Tsoy, Sergey; Bram, Yaron; Park, Jiwoon; Overbey, Eliah; Ryon, Krista; Haltom, Jeffrey; Singh, Urminder; Enguita, Francisco J; Zaksas, Victoria; Guarnieri, Joseph W; Topper, Michael; Wallace, Douglas C; Meydan, Cem; Baylin, Stephen; Meller, Robert; Muratani, Masafumi; Porterfield, D Marshall; Kaufman, Brett; Mori, Marcelo A; Walsh, Stephen B; Sigaudo-Roussel, Dominique; Mebarek, Saida; Bottini, Massimo; Marquette, Christophe A; Wurtele, Eve Syrkin; Schwartz, Robert E; Galeano, Diego; Mason, Christopher E; Grabham, Peter; Beheshti, Afshin.
Afiliación
  • McDonald JT; Department of Radiation Medicine, Georgetown University School of Medicine, Washington, D.C, USA.
  • Kim J; Department of Physiology, Biophysics and Systems Biology and the WorldQuant Initiative, Weill Cornell Medicine, New York, NY, USA.
  • Farmerie L; Vascular Medicine Institute at the University of Pittsburgh Department of Medicine, Pittsburgh, PA, USA.
  • Johnson ML; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, 15213, USA.
  • Trovao NS; Vascular Medicine Institute at the University of Pittsburgh Department of Medicine, Pittsburgh, PA, USA.
  • Arif S; Division of International Epidemiology and Population Studies, Fogarty International Center, National Institutes of Health, Bethesda, Maryland, USA.
  • Siew K; Center for Data-Driven Discovery in Biomedicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Tsoy S; Division of Neurosurgery, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
  • Bram Y; London Tubular Centre, Department of Renal Medicine, University College London, London, UK.
  • Park J; Division of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY, USA.
  • Overbey E; Division of Gastroenterology and Hepatology, Department of Medicine, Weill Cornell Medicine, New York, NY, USA.
  • Ryon K; Department of Physiology, Biophysics and Systems Biology and the WorldQuant Initiative, Weill Cornell Medicine, New York, NY, USA.
  • Haltom J; Department of Physiology, Biophysics and Systems Biology and the WorldQuant Initiative, Weill Cornell Medicine, New York, NY, USA.
  • Singh U; Department of Physiology, Biophysics and Systems Biology and the WorldQuant Initiative, Weill Cornell Medicine, New York, NY, USA.
  • Enguita FJ; The Center for Mitochondrial and Epigenomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA, 19104, USA.
  • Zaksas V; Bioinformatics and Computational Biology Program, Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 90011, USA.
  • Guarnieri JW; Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 1649-028, Lisboa, Portugal.
  • Topper M; Center for Translational Data Science, University of Chicago, Chicago, IL, 60637, USA.
  • Wallace DC; Clever Research Lab, Springfield, IL, 62704, USA.
  • Meydan C; The Center for Mitochondrial and Epigenomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA, 19104, USA.
  • Baylin S; Departments of Oncology and Medicine and the Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
  • Meller R; The Center for Mitochondrial and Epigenomic Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA, 19104, USA.
  • Muratani M; Department of Pediatrics, Division of Human Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA.
  • Porterfield DM; Department of Physiology, Biophysics and Systems Biology and the WorldQuant Initiative, Weill Cornell Medicine, New York, NY, USA.
  • Kaufman B; Departments of Oncology and Medicine and the Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins Medical Institutions, Baltimore, MD, USA.
  • Mori MA; Neuroscience Institute, Department of Neurobiology/ Department of Pharmacology and Toxicology, Morehouse School of Medicine, Atlanta, GA, 30310, USA.
  • Walsh SB; Transborder Medical Research Center, University of Tsukuba, Ibaraki, 305-8575, Japan.
  • Sigaudo-Roussel D; Department of Genome Biology, Institute of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan.
  • Mebarek S; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, 47907, USA.
  • Bottini M; Vascular Medicine Institute at the University of Pittsburgh Department of Medicine, Pittsburgh, PA, USA.
  • Marquette CA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, 15213, USA.
  • Wurtele ES; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas, Campinas, SP, Brazil.
  • Schwartz RE; Obesity and Comorbidities Research Center (OCRC), Universidade Estadual de Campinas, Campinas, SP, Brazil.
  • Galeano D; London Tubular Centre, Department of Renal Medicine, University College London, London, UK.
  • Mason CE; LBTI- UMR CNRS 5305, Université Claude Bernard Lyon 1, Lyon, France.
  • Grabham P; ICBMS, UMR5246, CNRS, INSA, CPE-Lyon, Université Claude Bernard Lyon 1, Villeurbanne, France.
  • Beheshti A; Department of Experimental Medicine, University of Rome Tor Vergata, 00133, Rome, Italy.
Nat Commun ; 15(1): 4825, 2024 Jun 11.
Article en En | MEDLINE | ID: mdl-38862542
ABSTRACT
Our previous research revealed a key microRNA signature that is associated with spaceflight that can be used as a biomarker and to develop countermeasure treatments to mitigate the damage caused by space radiation. Here, we expand on this work to determine the biological factors rescued by the countermeasure treatment. We performed RNA-sequencing and transcriptomic analysis on 3D microvessel cell cultures exposed to simulated deep space radiation (0.5 Gy of Galactic Cosmic Radiation) with and without the antagonists to three microRNAs miR-16-5p, miR-125b-5p, and let-7a-5p (i.e., antagomirs). Significant reduction of inflammation and DNA double strand breaks (DSBs) activity and rescue of mitochondria functions are observed after antagomir treatment. Using data from astronaut participants in the NASA Twin Study, Inspiration4, and JAXA missions, we reveal the genes and pathways implicated in the action of these antagomirs are altered in humans. Our findings indicate a countermeasure strategy that can potentially be utilized by astronauts in spaceflight missions to mitigate space radiation damage.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vuelo Espacial / Astronautas / Radiación Cósmica / MicroARNs Límite: Adult / Female / Humans / Male Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vuelo Espacial / Astronautas / Radiación Cósmica / MicroARNs Límite: Adult / Female / Humans / Male Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos