Torques within and outside the human spindle balance twist at anaphase.
J Cell Biol
; 223(9)2024 Sep 02.
Article
en En
| MEDLINE
| ID: mdl-38869473
ABSTRACT
At each cell division, nanometer-scale motors and microtubules give rise to the micron-scale spindle. Many mitotic motors step helically around microtubules in vitro, and most are predicted to twist the spindle in a left-handed direction. However, the human spindle exhibits only slight global twist, raising the question of how these molecular torques are balanced. Here, we find that anaphase spindles in the epithelial cell line MCF10A have a high baseline twist, and we identify factors that both increase and decrease this twist. The midzone motors KIF4A and MKLP1 are together required for left-handed twist at anaphase, and we show that KIF4A generates left-handed torque in vitro. The actin cytoskeleton also contributes to left-handed twist, but dynein and its cortical recruitment factor LGN counteract it. Together, our work demonstrates that force generators regulate twist in opposite directions from both within and outside the spindle, preventing strong spindle twist during chromosome segregation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cinesinas
/
Anafase
/
Microtúbulos
/
Huso Acromático
Límite:
Humans
Idioma:
En
Revista:
J Cell Biol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos