Use of a sample injection loop for an accurate measurement of particle number concentration by flow cytometry.
Anal Bioanal Chem
; 416(20): 4481-4490, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38926227
ABSTRACT
Flow cytometry plays a pivotal role in biotechnology by providing quantitative measurements for a wide range of applications. Nonetheless, achieving precise particle quantification, particularly without relying on counting beads, remains a challenge. In this study, we introduce a novel exhaustive counting method featuring a sample loop-based injection system that delivers a defined sample volume to a detection system to enhance quantification in flow cytometry. We systematically assess the performance characteristics of this system with micron-sized polystyrene beads, addressing issues related to sample introduction, adsorption, and volume measurement. Results underscore the excellent analytical performance of the proposed method, characterized by high linearity and repeatability. We compare our approach to counting bead-based measurements, and while an approximate bias value was observed, the measured values were found to be similar between the methods, demonstrating its comparability and reliability. This method holds great promise for improving the accuracy and precision of particle quantification in flow cytometry, with implications for various fields including healthcare and environmental monitoring.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Tamaño de la Partícula
/
Poliestirenos
/
Citometría de Flujo
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2024
Tipo del documento:
Article