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The Characterization of a Novel PrMADS11 Transcription Factor from Pinus radiata Induced Early in Bent Pine Stem.
Méndez, Tamara; Guajardo, Joselin; Cruz, Nicolás; Gutiérrez, Rodrigo A; Norambuena, Lorena; Vega, Andrea; Moya-León, María A; Herrera, Raúl.
Afiliación
  • Méndez T; Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.
  • Guajardo J; Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.
  • Cruz N; Facultad de Ciencias Agrarias y Forestales, Universidad Técnica Estatal de Quevedo, Quevedo 120313, Ecuador.
  • Gutiérrez RA; Millennium Institute Center for Genome Regulation, Millennium Institute for Integrative Biology, Instituto de Ecología y Biodiversidad, Facultad Ciencias Biológicas, P. Universidad Católica de Chile, Avda, Libertador Bernardo O'Higgins 340, Santiago 8331150, Chile.
  • Norambuena L; Plant Molecular Biology Centre, Department of Biology, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Santiago 7750000, Chile.
  • Vega A; Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Peñalolen 7940000, Chile.
  • Moya-León MA; Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.
  • Herrera R; Instituto de Ciencias Biológicas, Universidad de Talca, Av. Lircay s/n, Talca 3465548, Chile.
Int J Mol Sci ; 25(13)2024 Jun 30.
Article en En | MEDLINE | ID: mdl-39000352
ABSTRACT
A novel MADS-box transcription factor from Pinus radiata D. Don was characterized. PrMADS11 encodes a protein of 165 amino acids for a MADS-box transcription factor belonging to group II, related to the MIKC protein structure. PrMADS11 was differentially expressed in the stems of pine trees in response to 45° inclination at early times (1 h). Arabidopsis thaliana was stably transformed with a 35SPrMADS11 construct in an effort to identify the putative targets of PrMADS11. A massive transcriptome analysis revealed 947 differentially expressed genes 498 genes were up-regulated, and 449 genes were down-regulated due to the over-expression of PrMADS11. The gene ontology analysis highlighted a cell wall remodeling function among the differentially expressed genes, suggesting the active participation of cell wall modification required during the response to vertical stem loss. In addition, the phenylpropanoid pathway was also indicated as a PrMADS11 target, displaying a marked increment in the expression of the genes driven to the biosynthesis of monolignols. The EMSA assays confirmed that PrMADS11 interacts with CArG-box sequences. This TF modulates the gene expression of several molecular pathways, including other TFs, as well as the genes involved in cell wall remodeling. The increment in the lignin content and the genes involved in cell wall dynamics could be an indication of the key role of PrMADS11 in the response to trunk inclination.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Regulación de la Expresión Génica de las Plantas / Pinus Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Chile

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Regulación de la Expresión Génica de las Plantas / Pinus Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Chile