Regulation of AreA on lipid biosynthesis under different nitrogen sources and C/N ratios in the model oleaginous fungus Mucor circinelloides.
Biochim Biophys Acta Mol Cell Biol Lipids
; 1869(7): 159537, 2024 Oct.
Article
en En
| MEDLINE
| ID: mdl-39032627
ABSTRACT
Mucor circinelloides has been exploited as model filamentous fungi for studies of genetic manipulation of lipogenesis. It is widely recognized that lipid accumulation is increased when there is a lack of nitrogen source in oleaginous microorganism. Nitrogen metabolism in filamentous fungi is a complex process that can be regulated by the global nitrogen regulator AreA. In this study, we cultivated the areA-knockout and -overexpression strains obtained in our previous study, using 20 different nitrogen sources. It emerged that the disruption of AreA in M. circinelloides reduced its sensitivity to nitrogen availability, resulting in increased lipid synthesis. Specially, the areA-knockout strain was unable to fully utilize many nitrogen sources but the ammonium and glutamate. We continued to investigate lipid production at different molar C/N ratios using glucose as sole carbon source and ammonium sulfate as sole nitrogen source, of which the high C/N ratios activate high lipid accumulation. By comparing the experimental results with transcriptional analysis, we were able to identify the optimal process conditions suitable for lipid accumulation and potential targets for future metabolic engineering.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Fúngicas
/
Carbono
/
Mucor
/
Nitrógeno
Idioma:
En
Revista:
Biochim Biophys Acta Mol Cell Biol Lipids
/
Biochim. biophys. acta, Mol. cell biol. lipids (Online)
/
Biochimica and biophysica acta. Molecular and cell biology of lipids (Online)
Año:
2024
Tipo del documento:
Article
País de afiliación:
China