Protocol for characterizing non-genetic heterogeneity and expression dynamics of surface proteins in mouse muscle stem cells using flow cytometry.
STAR Protoc
; 5(3): 103216, 2024 Jul 27.
Article
en En
| MEDLINE
| ID: mdl-39068657
ABSTRACT
Here, we present a protocol for investigating the non-genetic heterogeneity of membrane proteins expression within murine muscle stem cell (MuSC) population isolated from injured skeletal muscles. We describe a protocol that employs flow cytometry technology to detect variations in membrane CRIPTO protein levels and ensure measurements standardization. We detail steps for muscle digestion, bulk muscle cell staining, and phenotypic analysis. This approach allows for the identification of MuSC fractions with distinct phenotypic and functional properties. For complete details on the use and execution of this protocol, please refer to Guardiola et al.1.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article