Chemical and physicochemical characterization of CB-1a, an allergenic fraction isolated from castor bean (Ricinus communis L.).
An Acad Bras Cienc
; 56(3): 323-31, 1984 Sep.
Article
en En
| MEDLINE
| ID: mdl-6508026
ABSTRACT
CB-1A chemical characterization comprised the determination of nitrogen (Kjeldhal 15.5% amino acid composition 17.5%), total (6.8%) and reducing (1.6%) carbohydrates, amino acid composition (Lys4, His1, Arg11, Asp3, Thr1, Ser7, Glu16, Pro2, Gly5, Ala3, Cys6, Val3, Met1, Ile3, Leu4, Tyr1, Phe1; 82 residues). Its behaviour under several TCA concentrations, ultraviolet absorption of native and oxidized CB-1A under several solvents as well as its enzymatic susceptibility were explored. Physicochemical parameters such as MW (protein moiety - composition 9.642 Da, column 9.431 Da glycoprotein-10.345) Da; E1%1cm,220mm = 49.7 E1%1cm,595mm = 29.7 partial specific volume (anhydrous 0.703 cm3/g; hydrated; 0.924 cm3/g); Stokes radius (15.1 A); hydration water (22%) and frictional ratio (110) were determined. Calculated limit molecular dimensions (semi-axis of revolution 11.4 to 19.8 A) and equatorial radius (13.2 to 17.5 A suggest dendency to sphericity. CB-1A showed allergenicity by PCA. Fourteen components were detected by IEF.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plantas Tóxicas
/
Ricinus
/
Alérgenos
/
Glicoproteínas
/
Ricinus communis
Límite:
Animals
Idioma:
En
Revista:
An Acad Bras Cienc
Año:
1984
Tipo del documento:
Article