Studies on histone oligomers. II. Reconstitution of heterotype histone oligomers and pH dependency of oligomer formation.
J Biochem
; 88(3): 783-8, 1980 Sep.
Article
en En
| MEDLINE
| ID: mdl-7419522
ABSTRACT
Histone oligomers were reconstituted by annealing from dissociated calf thymus whole histones in 2 M NaCl-5 M urea. The products were fractionated in terms of solubility in ammonium sulfate solution. The oligomers formed at pH 5 were heterotype histone oligomers (H2A . H2B . H3 . H4)n at high ionic strength, and heterotype histone tetramer (H2A . H2B . H3 . H4) at low ionic strength. The pH dependency of oligomer formation was determined. Heterotype oligomers were formed at pH 4-6 and homotype oligomers at pH 7-9.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Histonas
Límite:
Animals
Idioma:
En
Revista:
J Biochem
Año:
1980
Tipo del documento:
Article