Selection of antigenic and immunogenic mimics of hepatitis C virus using sera from patients.
J Immunol
; 156(11): 4504-13, 1996 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-8666827
Using sera from hepatitis C virus (HCV)-infected patients and noninfected subjects to screen random peptide libraries displayed on phage, we selected peptides specifically reacting with sera from infected patients. These phage- borne peptides were shown to mimic distinct HCV determinants. They detected in all cases the presence of anti-HCV Abs in a large panel of patients' sera, thus demonstrating the high sensitivity of the selected peptides as diagnostic markers. In addition, this diagnostic approach allowed a detailed characterization of the individual humoral response to viral infection. Phage-displayed HCV mimics were substitutes for the authentic HCV epitopes in inducing a strong specific response against HCV when used as immunogens in mice. These results support the search for HCV mimics with the potential to elicit a protective immune response as leads for the development of a mimotope-based vaccine against viral infection.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Hepatitis C
/
Hepacivirus
/
Imitación Molecular
/
Antígenos Virales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol
Año:
1996
Tipo del documento:
Article
País de afiliación:
Italia