Fate of Neutrophils during the Recovery Phase of Ischemia/Reperfusion Induced Acute Kidney Injury
Journal of Korean Medical Science
; : 1616-1625, 2017.
Article
en En
| WPRIM
| ID: wpr-14439
Biblioteca responsable:
WPRO
ABSTRACT
Effective clearance of inflammatory cells is required for resolution of inflammation. Here, we show in vivo evidence that apoptosis and reverse transendothelial migration (rTEM) are important mechanisms in eliminating neutrophils and facilitating recovery following ischemia/reperfusion injury (IRI) of the kidney. The clearance of neutrophils was delayed in the Bax knockout (KO)BM → wild-type (WT) chimera in which bone marrow derived cells are partially resistant to apoptosis, compared to WTBM → WT mice. These mice also showed delayed functional, histological recovery, increased tissue cytokines, and accelerated fibrosis. The circulating intercellular adhesion molecule-1 (ICAM-1)+ Gr-1+ neutrophils displaying rTEM phenotype increased during the recovery phase and blockade of junctional adhesion molecule-C (JAM-C), a negative regulator of rTEM, resulted in an increase in circulating ICAM-1+ neutrophils, faster resolution of inflammation and recovery. The presence of Tamm-Horsfall protein (THP) in circulating ICAM-1+ neutrophils could suggest that they are derived from injured kidneys. In conclusion, we suggest that apoptosis and rTEM are critically involved in the clearance mechanisms of neutrophils during the recovery phase of IRI.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Fenotipo
/
Médula Ósea
/
Fibrosis
/
Quimera
/
Citocinas
/
Apoptosis
/
Molécula 1 de Adhesión Intercelular
/
Lesión Renal Aguda
/
Uromodulina
/
Migración Transendotelial y Transepitelial
Límite:
Animals
Idioma:
En
Revista:
Journal of Korean Medical Science
Año:
2017
Tipo del documento:
Article