Inhibition of MCF-7/ADR cells by DOX-loaded pluronic-attached PAMAM dendrimer conjugate / 药学学报
Acta Pharmaceutica Sinica
; (12): 1188-1193, 2014.
Article
en Zh
| WPRIM
| ID: wpr-299148
Biblioteca responsable:
WPRO
ABSTRACT
Pluronic modified polyamidoamine (PAMAM) conjugate (PF127-PAMAM) was prepared and the inhibiting effect of MDR against MCF-7/ADR was investigated with doxorubicin (DOX) as model drug. 1H NMR and FTIR spectra showed that the conjugate was synthesized successfully. Element analysis accurately measured that 27.63% amino of per PAMAM was modified by pluronic (PAMAM : PF127, 1 : 35.37 mole ratio). PF127-PAMAM showed an increased size and a reduced zeta potential compared to PAMAM. PF127-PAMAM had lower hemolytic toxicity and cytotoxicity due to the reduced zeta potential and the protection of PF127. Each PF127-PAMAM molecular could load 19.58 DOX molecules, and the complex exhibited sustained and pH-sensitive release behavior. PF127-PAMAM/DOX exhibited weaker cytotoxicity than free DOX in MCF-7 cells; while the complex showed much stronger reverse effect of drug resistance in MCF-7/ADR cells, and resistance reversion index (RRI) was as high as 33.15.
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Farmacología
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Doxorrubicina
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Poloxámero
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Dendrímeros
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Células MCF-7
Límite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2014
Tipo del documento:
Article