Identification of biomarkers for endometriosis using clinical proteomics / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 520-527, 2015.
Article
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| WPRIM
| ID: wpr-357968
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods.</p><p><b>METHODS</b>We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS.</p><p><b>RESULTS</b>We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively.</p><p><b>CONCLUSIONS</b>ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.</p>
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WPRIM
Asunto principal:
Péptidos
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Sangre
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Biomarcadores
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Proteómica
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Diagnóstico
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Endometriosis
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Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
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Adult
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Female
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Humans
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2015
Tipo del documento:
Article