Involvement of Heme Oxygenase-1 in Orexin-A-induced Angiogenesis in Vascular Endothelial Cells
The Korean Journal of Physiology and Pharmacology
; : 327-334, 2015.
Article
en En
| WPRIM
| ID: wpr-727366
Biblioteca responsable:
WPRO
ABSTRACT
The cytoprotective enzyme heme oxygenase-1 (HO-1) influences endothelial cell survival, proliferation, inflammatory response, and angiogenesis in response to various angiogenic stimuli. In this study, we investigate the involvement of HO-1 in the angiogenic activity of orexin-A. We showed that orexin-A stimulates expression and activity of HO-1 in human umbilical vein endothelial cells (HUVECs). Furthermore, we showed that inhibition of HO-1 by tin (Sn) protoporphryin-IX (SnPP) reduced orexin-A-induced angiogenesis in vivo and ex vivo. Orexin-A-stimulated endothelial tube formation and chemotactic activity were also blocked in SnPP-treated vascular endothelial cells. Orexin-A treatment increased the expression of nuclear factor erythroid-derived 2 related factor 2 (Nrf2), and antioxidant response element (ARE) luciferase activity, leading to induction of HO-1. Collectively, these findings indicate that HO-1 plays a role as an important mediator of orexin-A-induced angiogenesis, and provide new possibilities for therapeutic approaches in pathophysiological conditions associated with angiogenesis.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Estaño
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Células Endoteliales
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Hemo-Oxigenasa 1
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Células Endoteliales de la Vena Umbilical Humana
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Elementos de Respuesta Antioxidante
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Orexinas
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Luciferasas
Idioma:
En
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2015
Tipo del documento:
Article