[Electrofocusing of immunoglobulin G that reacts positively in the sedimentation reaction for cancer]. / Elektrofokusuvannia imunohlobulinu G, shcho pozytyvno reahuie v osadovii reaktsiï na rak

ABSTRACT

Immunoglobulin G from the serum of patients with myeloma and positively reacting in the sedimentary test for cancer (PPR-STC) was purified by DEAE-Spehadex A-50 and KM-cellulose chromotography and studied by the method of isoelectrofocusing; Application of 1% ampholine within the pH gradient 3.0-10.0 shows the difference between the isoelectric spectra of immunoglobulin G from the donor and from a patient with myeloma. The PPR-STC isoelectric point located in the zone of pH 8.4 is determined. No subfraction with pH 8.4 is observed in the fraction of the donor immunoglobulin G.