The main regulatory region in the murine PSP gene is a parotid gland enhancer.
Transgenic Res
; 7(6): 413-20, 1998 Nov.
Article
em En
| MEDLINE
| ID: mdl-10341449
ABSTRACT
The murine PSP gene is expressed at a high-level in the parotid glands. To extend the knowledge of parotid gland expression and develop tools for expression of heterologous proteins in this tissue, the regulation of the PSP gene was studied using transgenic mice. High-level parotid gland expression of the PSP gene was indicated to depend on a novel regulatory region situated between -8.0 and -6.5 kb. Together with previous results this indicates that the main regulatory elements in the PSP gene are situated between -8.0 to -3.1 kb. This region was shown to activate a heterologus SV40 early promoter in the parotid glands of transgenic mice, suggesting that the PSP gene is controlled by enhancer sequences. A novel Psp derived 9.7 kb parotid gland expression cassette, Lama IV, carrying all known regulatory regions in the PSP gene was expressed at high-levels in the parotid glands and should prove highly useful for expression of heterologous proteins in the saliva of transgenic mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glândula Parótida
/
Proteínas e Peptídeos Salivares
/
Regulação da Expressão Gênica
/
Elementos Facilitadores Genéticos
/
Transgenes
Limite:
Animals
Idioma:
En
Revista:
Transgenic Res
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Dinamarca