Identification of human TGF-beta1 signal (leader) sequence polymorphisms by PCR-RFLP.
J Immunol Methods
; 234(1-2): 117-22, 2000 Feb 03.
Article
em En
| MEDLINE
| ID: mdl-10669776
ABSTRACT
Links between disease susceptibility and genetically determined variation in human cytokine expression have recently been described. This has led to a demand for simple methods of identifying cytokine gene polymorphisms of potential clinical relevance. Here, we describe a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for identifying two human transforming growth factor beta1 (TGF-beta1) signal (leader) sequence polymorphisms, T869C (Leu10Pro) and G915C (Arg25Pro). This permits simple and robust identification of TGF-beta1 leader sequence genotypes and demonstrates the physical linkage in cis between T869C (Leu10Pro) and G915C (Arg25Pro). The method does not require previously genotyped standards. The efficacy of enzyme digestion is internally controlled by the presence of conserved restriction sites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Sinais Direcionadores de Proteínas
/
Fator de Crescimento Transformador beta
Tipo de estudo:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Reino Unido