Migration behavior and separation of benzenediamines, aminophenols and benzenediols by capillary zone electrophoresis.
J Chromatogr A
; 871(1-2): 357-66, 2000 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-10735316
ABSTRACT
The migration behavior and separation of five benzendiamines, five aminophenols and three benzenediols were investigated in capillary zone electrophoresis. The results indicate that benzendiamines and aminophenols are optimally separated with a phosphate buffer at pH 5, whereas benzenediol isomers are best separated at pH about 12. The addition of surfactant monomers of tetradecyltrimethylammonium bromide to a phosphate buffer at pH 5 under the conditions of reversed electroosmotic flow is effective for separating these dye intermediates, except for the separation of 1,2-benzenediol from 1,3-benzenediol. The addition of sodium tetraborate as an electrolyte modifier is effective in the separation of 1,2-benzenediol from 1,3-benzenediol, but the latter comigrates with the 1,4-benzenediol isomer at pH 5.0. The electrophoretic mobility of ionized analytes can be described with Offord's equation, and the migration order depends on their ratios of charge to mass. In addition, the pKa values of these analytes in 50 mM phosphate buffer are reported.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Derivados de Benzeno
/
Eletroforese Capilar
/
Aminofenóis
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2000
Tipo de documento:
Article