Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential.
Cancer Lett
; 155(2): 153-61, 2000 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-10822130
ABSTRACT
We present here a new cell line, NOR-P1, established from a metastatic subcutaneous tumor of a patient with pancreatic cancer. The cells show rapid growth in culture with a doubling time of 16 h and high migration activity. Genetic and molecular analyses revealed high telomerase activity and a mutation in the K-ras oncogene. Of particular interest, the cells express markedly elevated mRNA levels of angiogenic factors, vascular endothelial growth factor and platelet-derived growth factor, as well as other tumor growth-related factors. Subcutaneous transplantation of the NOR-P1 cells into nude mice formed solid, hemorrhagic tumors which were histologically diagnosed as adenocarcinoma with dense blood vessels and severe extravasation of blood. Furthermore, when NOR-P1 cell suspension was injected directly into the pancreas of nude mice, the cells grew rapidly to form intra-pancreatic tumors associated with liver metastases and peritoneal dissemination that resulted in cachexia and subsequent death. These properties suggest that NOR-P1 is an aggressive pancreatic cancer cell line with a high metastatic potential and may serve as a useful experimental model for studying tumor angiogenesis and metastasis of pancreatic cancer.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Células Tumorais Cultivadas
/
Neovascularização Patológica
Tipo de estudo:
Prognostic_studies
Limite:
Aged
/
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Cancer Lett
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão