Pig cloning by microinjection of fetal fibroblast nuclei.
Science
; 289(5482): 1188-90, 2000 Aug 18.
Article
em En
| MEDLINE
| ID: mdl-10947985
ABSTRACT
Pig cloning will have a marked impact on the optimization of meat production and xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of porcine (Sus scrofa) fetal fibroblasts into enucleated oocytes, and development was induced by electroactivation. The transfer of 110 cloned embryos to four surrogate mothers produced an apparently normal female piglet. The clonal provenance of the piglet was indicated by her coat color and confirmed by DNA microsatellite analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Suínos
/
Clonagem de Organismos
/
Técnicas de Transferência Nuclear
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Science
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Japão