Simultaneous stochastic sensing of divalent metal ions.
Nat Biotechnol
; 18(9): 1005-7, 2000 Sep.
Article
em En
| MEDLINE
| ID: mdl-10973225
ABSTRACT
Stochastic sensing is an emerging analytical technique that relies upon single-molecule detection. Transmembrane pores, into which binding sites for analytes have been placed by genetic engineering, have been developed as stochastic sensing elements. Reversible occupation of an engineered binding site modulates the ionic current passing through a pore in a transmembrane potential and thereby provides both the concentration of an analyte and, through a characteristic signature, its identity. Here, we show that the concentrations of two or more divalent metal ions in solution can be determined simultaneously with a single sensor element. Further, the sensor element can be permanently calibrated without a detailed understanding of the kinetics of interaction of the metal ions with the engineered pore.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processos Estocásticos
/
Eletroquímica
/
Íons
/
Metais
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Nat Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2000
Tipo de documento:
Article
País de afiliação:
Estados Unidos