Green plant photosystem I binds light-harvesting complex I on one side of the complex.
Biochemistry
; 40(4): 1029-36, 2001 Jan 30.
Article
em En
| MEDLINE
| ID: mdl-11170425
ABSTRACT
We report a structural characterization by electron microscopy of green plant photosystem I solubilized by the mild detergent n-dodecyl-alpha-D-maltoside. It is shown by immunoblotting that the isolated complexes contain all photosystem I core proteins and all peripheral light-harvesting proteins. The electron microscopic analysis is based on a large data set of 14 000 negatively stained single-particle projections and reveals that most of the complexes are oval-shaped monomers. The monomers have a tendency to associate into artificial dimers, trimers, and tetramers in which the monomers are oppositely oriented. Classification of the dimeric complexes suggests that some of the monomers lack a part of the peripheral antenna. On the basis of a comparison with projections from trimeric photosystem I complexes from cyanobacteria, we conclude that light-harvesting complex I only binds to the core complex at the side of the photosystem I F/J subunits and does not cause structural hindrances for the type of trimerization observed in cyanobacterial photosystem I.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Spinacia oleracea
/
Complexo de Proteínas do Centro de Reação Fotossintética
Idioma:
En
Revista:
Biochemistry
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Holanda