Calibration curves for biological dosimetry by fluorescence in situ hybridisation.
Radiat Prot Dosimetry
; 94(4): 335-45, 2001.
Article
em En
| MEDLINE
| ID: mdl-11499437
ABSTRACT
Dose-response curves were measured for the induction of chromosomal aberrations in peripheral blood lymphocytes after acute exposure in vitro to 60Co gamma rays. Blood was obtained from four different healthy donors, and chromosomes were either observed at metaphase, following colcemid accumulation, or prematurely condensed by calyculin A. Cells were analysed in three different Italian laboratories. Chromosomes 1, 2, and 4 were painted, and simple-type interchanges between painted and non-painted chromosomes were scored in cells exposed in the dose range 0.1-3.0 Gy. The chemical-induced premature chromosome condensation method was also used combined with chromosome painting (chromosome 4 only) to determine calibration curves for high dose exposures (up to 20 Gy X rays). Calibration curves described in this paper will be used in our laboratories for biological dosimetry by fluorescence in situ hybridisation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos
/
Aberrações Cromossômicas
/
Hibridização in Situ Fluorescente
/
Transtornos Cromossômicos
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Radiat Prot Dosimetry
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Itália