Differential expression of the isoforms for the monocyte chemoattractant protein-1 receptor, CCR2, in monocytes.
Biochem Biophys Res Commun
; 290(1): 73-80, 2002 Jan 11.
Article
em En
| MEDLINE
| ID: mdl-11779135
ABSTRACT
Two isoforms of human CCR2, the receptor for monocyte chemoattractant protein-1 (MCP-1), have been identified but their relative expression in monocytes and contribution to inflammatory responses mediated by MCP-1 remain uncertain. All available information on CCR2 expression is based on mRNA data because isoform-specific antibodies were not available until now. To analyze the relative expression of each isoform, we made two antibodies that specifically recognized CCR2A and CCR2B. Examination of receptor protein with these isoform-specific antibodies showed that the total expression of CCR2B in monocytes was about 10-fold higher than that of CCR2A with an equal distribution between the cell surface and intracellular pools. A detailed analysis using purified plasma membranes demonstrated that about 90% of all CCR2 on the cell surface were composed of CCR2B. The relatively abundant expression of CCR2B on the cell surface suggests a principal role of this isoform as a mediator of monocyte responses to MCP-1 in inflammation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de Quimiocinas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos