Genetic diversity of Colombian sylvatic Trypanosoma cruzi isolates revealed by the ribosomal DNA.
Mem Inst Oswaldo Cruz
; 97(6): 877-80, 2002 Sep.
Article
em En
| MEDLINE
| ID: mdl-12386714
ABSTRACT
American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis. The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
Variação Genética
/
DNA Ribossômico
/
Insetos Vetores
Limite:
Animals
País/Região como assunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Colômbia