HBV C promoter Sp1 binding sequence functionally substitutes for the yeast ARS1 ABF1 binding site.
DNA Cell Biol
; 21(10): 737-42, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12443543
ABSTRACT
Transcriptional factors have been implicated in eukaryotic DNA replication. We have studied the potential function of a viral promoter sequence in DNA replication. The hepatitis B virus (HBV) pregenomic promoter is regulated by two enhancers and cis-elements. The G-C rich region between 1734-1754 nt, which contains two SP1 binding sites, is necessary for transcription origin and HBV replication. We found that the Abf1-binding B3 element in yeast ARS1 can be functionally replaced by the viral Sp1-binding DNA sequence, which activates transcription from the HBV C promoter. Further, yeast RAP1 bound to the viral Sp1 binding sites in vitro. These results suggest that RAP1 binds to the Sp1 binding sites and stimulates yeast DNA replication.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Fatores de Transcrição
/
Vírus da Hepatite B
/
Fator de Transcrição Sp1
/
Proteínas de Saccharomyces cerevisiae
/
Proteínas de Ligação a DNA
Limite:
Humans
Idioma:
En
Revista:
DNA Cell Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
China