[Measurement of baicalin contents by HPLC].

ABSTRACT

OBJECTIVE: To compare the contents of baicalin in the raw medical material and the different prepared medical materials of Scutellaria baicalensis. METHOD: The contents of baicalin were determined by HPLC. Chromatographic conditions included Hypersil C18 column and the mobile phase consisting of a mixture of methanol-0.04% phosphoric acid (4654). Baicalin was detected at 280 nm. The standard curve was linear in the range of 0.0280-4.6700 micrograms with correlation coefficient 1.0000. The average recovery of baicalin was 101.22% with RSD = 2.54% (n = 5). RESULT: The contents of baicalin were 6.80% in raw material, 6.00% and 6.73% in the different prepared medical materials of S. baicalensis respectively. CONCLUSION: The two different kinds of preparing methods can be used to the raw material of S. baicalensis.