[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 20(4): 345-7, 2003 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-12903049
ABSTRACT
OBJECTIVE:
To establish a rapid and convenient method of reverse dot blot (RDB) analysis for detecting the point mutations of non-deletion alpha-thalassemia in Chinese.METHODS:
Label biotin to primers and amplify human alpha2 globin gene selectively, then hybridize PCR products with a set of oligonucleotide probes immobilized on strips, and develop colour to detect non-deletion alpha-thalassemia defects.RESULTS:
The PCR system using biotin-labeled primers could specifically amplify a 1085 bp fragment of human alpha2 globin gene which encompasses all six alpha-thalassemia mutations. After being hybridized with sequence-specific oligonucleotide probes and colour development, it could simultaneously identify all six types of non-deletion alpha-thalassemias encountered in Chinese.CONCLUSION:
This method does not need semi-nested PCR, and the products amplified by biotinylated primers can be used directly to hybridize with the probes on strips under the identical conditions of hybridization. So, it is a specific and multiplex detection assay for screening non-deletion alpha-thalassemia defects in Chinese.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Talassemia alfa
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Assunto da revista:
GENETICA MEDICA
Ano de publicação:
2003
Tipo de documento:
Article