Formation of glucosylceramide and sterol glucoside by a UDP-glucose-dependent glucosylceramide synthase from cotton expressed in Pichia pastoris.
FEBS Lett
; 553(3): 365-9, 2003 Oct 23.
Article
em En
| MEDLINE
| ID: mdl-14572652
ABSTRACT
In plants, glucosylceramide (GlcCer) biosynthesis is poorly understood. Previous investigations suggested that sterol glucoside (SG) acts as the actual glucose donor for the plant GlcCer synthase (GCS). We addressed this question by generating a Pichia pastoris double mutant devoid of GlcCer and SG. This mutant was used for heterologous expression of the plant GCS. The activity of the GCS resulted in the accumulation of GlcCer and, surprisingly, a small proportion of SG. The synthesis of GlcCer in the transformed double mutant shows that the GCS is SG-independent, while the detection of SG suggests that in addition to the sterol glucosyltransferase, also the GCS may contribute in planta to SG biosynthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Esteróis
/
Uridina Difosfato Glucose
/
Gossypium
/
Glucosídeos
/
Glucosilceramidas
/
Glucosiltransferases
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Alemanha