Cloning and mutation analysis of ZFP276 as a candidate tumor suppressor in breast cancer.

Wong, Jasmine C Y; Gokgoz, Nalan; Alon, Noa; Andrulis, Irene L; Buchwald, Manuel

Wong, Jasmine C Y; Gokgoz, Nalan; Alon, Noa; Andrulis, Irene L; Buchwald, Manuel.

Afiliação

Wong JCY; Program in Genetics and Genomics Biology, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1X8, Canada. jcywong@sickkids.ca.
Gokgoz N; Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada. jcywong@sickkids.ca.
Alon N; Fred A. Litwin Centre for Cancer Genetics, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5, Canada.
Andrulis IL; Program in Genetics and Genomics Biology, Research Institute, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, M5G 1X8, Canada.
Buchwald M; Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.

J Hum Genet ; 48(12): 668-671, 2003.

Article em En | MEDLINE | ID: mdl-14605947

ABSTRACT

ABSTRACT

Loss of heterozygosity (LOH) involving chromosome 16q23.4 occurs frequently in breast tumors, which suggests that this region may contain a tumor suppressor gene. Since ZFP276 is located in this region, we have therefore cloned and performed mutation analysis of its coding region in 70 breast tumors. One silent polymorphism and two alterations predicted to result in amino acid changes were detected. Absence of the wild-type allele in tumors carrying the E530D variant suggests a possible role for this change in tumorigenesis.

Assuntos

Neoplasias da Mama/genética; Genes Supressores de Tumor; Polimorfismo Genético; Proteínas Supressoras de Tumor/genética; Alelos; Sequência de Aminoácidos; Northern Blotting; Neoplasias da Mama/patologia; Cromossomos Humanos Par 16; Clonagem Molecular; Análise Mutacional de DNA; DNA Complementar/metabolismo; Feminino; Humanos; Modelos Genéticos; Dados de Sequência Molecular; Proteínas Nucleares; Polimorfismo Conformacional de Fita Simples; RNA Mensageiro/metabolismo; Homologia de Sequência de Aminoácidos; Dedos de Zinco

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo Genético / Neoplasias da Mama / Genes Supressores de Tumor / Proteínas Supressoras de Tumor Tipo de estudo: Prognostic_studies Limite: Female / Humans Idioma: En Revista: J Hum Genet Assunto da revista: GENETICA MEDICA Ano de publicação: 2003 Tipo de documento: Article País de afiliação: Canadá

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