Separation, identification, and quantification of selected withanolides in plant extracts of Withania somnifera by HPLC-UV(DAD)--positive ion electrospray ionisation-mass spectrometry.
J Sep Sci
; 27(7-8): 541-6, 2004 May.
Article
em En
| MEDLINE
| ID: mdl-15335037
ABSTRACT
This paper describes a method for separation, identification, and quantification of selected withanolides in Withania somnifera plant extracts by HPLC-UV(DAD)-Mass Spectrometry (HPLC-MS). Withaferin-A (WS-3), 12-deoxywithastramonolide (WS-12DS), Withanolide A (WS-1), and Withanone (WS-2) were used as external standards. The compounds were isolated from Withania somnifera by repeated column chromatography of the root extract and their identity was established by 1H- and 13C-NMR and mass spectral data. The compounds were chromatographed on a Merck (250 x 4.6 mm ID, 5 microm) column and analyzed by Electrospray Ionization on a mass spectrometer in Selected Ion Mode (SIM). For quantification, [M + Na]+ ions were monitored. Linear calibration curves were obtained in the concentration range of 1.50 microg/mL to 6.5 microg/mL. The method was applied successfully to the detection and quantification of the said withanolides in a number of samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Withania
/
Ergosterol
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Índia