Serum and erythocyte argininosuccinate lyase assay by NADH fluorescence generated from formed fumarate.
Clin Chem
; 21(2): 230-4, 1975 Feb.
Article
em En
| MEDLINE
| ID: mdl-163157
ABSTRACT
Measurement of argininosuccinase (I; EC 4.3.2.1) activity is useful in following the course of disease in hepatitis and in screening for the genetic defect, argininosuccinic aciduria. Methodology is proposed for a novel procedure for the determination of I in serum and erythrocytes. In the procedure, fumarate, generated in the reaction, is assayed by conversion to malate with fumarase, determining the malate enzymatically with malate dehydrogenase, and estimating the NADH formed spectrofluorometrically. By this procedure, the enzyme activity in serum from normal individuals is less than 11 mumol/liter of erthrocytes/per hour. The correlation coefficient between results by this method and by the colorimetric method, which measures the arginine generated in the reaction, is +0.97 for serum and +0.98 for erythrocytes. The proposed procedure has a relatively low initial blank, requires less serum, and is completed faster.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eritrócitos
/
Liases
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Clin Chem
Assunto da revista:
QUIMICA CLINICA
Ano de publicação:
1975
Tipo de documento:
Article