Enhancing the synthetic utility of aldolase antibody 38C2.
Bioorg Med Chem Lett
; 16(4): 807-10, 2006 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-16321532
ABSTRACT
Three-phase partitioning (TPP) treated aldolase antibody 38C2 was evaluated for aldol reaction between p-nitrobenzaldehyde and acetone to give 4-(4'-nitrophenyl)-4-hydroxy-2-butanone. While TPP-treated 38C2 transformed 65% of p-nitrobenzaldehyde, the untreated 38C2 gave only 24% transformation in 18 h at 25 degrees C. However, since TPP-treated 38C2 also gave an additional (unidentified) product, its synthetic utility was limited. Crosslinked aggregate of 38C2, however, gave the biocatalyst which gave a single product and could be reused at 40 degrees C five times without loss of activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Butanonas
/
Fragmentos Fab das Imunoglobulinas
/
Frutose-Bifosfato Aldolase
Idioma:
En
Revista:
Bioorg Med Chem Lett
Assunto da revista:
BIOQUIMICA
/
QUIMICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Índia