Analysis of genes dominantly expressed in rat cerebral endothelial cells using suppression subtractive hybridization.
J Atheroscler Thromb
; 12(6): 330-7, 2005.
Article
em En
| MEDLINE
| ID: mdl-16394617
ABSTRACT
In mammals, a fully developed, highly branched vascular system specialized for each particular organ or tissue is essential for obtaining metabolic nutrients supply. The formation of a blood-brain barrier that protects against environmental insults is a distinguishing feature of the brain's vascular system. Since this is accomplished by cerebral endothelial cells (CECs), we analyzed the genes specifically and/or dominantly expressed in rat CECs using Suppression Subtractive Hybridization (SSH). We found 39 genes specifically and/or dominantly expressed in CECs. 24 genes of known function (thrombospondin-2, vimentin, etc.), 13 genes of known sequence but unknown function including 7 of ESTs (SNERG1, rat GPCR, etc.), and 2 novel genes. The physiological significance of these genes in CECs has been under investigation. SSH is useful for identifying genes regulated in an organ-specific manner in cells such as CECs to obtain clarification of their physiological roles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Endotélio Vascular
/
Perfilação da Expressão Gênica
/
Genes Dominantes
Limite:
Animals
Idioma:
En
Revista:
J Atheroscler Thromb
Assunto da revista:
ANGIOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Japão