Lipid lateral diffusion in bilayers with phosphatidylcholine, sphingomyelin and cholesterol. An NMR study of dynamics and lateral phase separation.
Chem Phys Lipids
; 141(1-2): 179-84, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16580657
ABSTRACT
Pulsed field gradient (pfg)-NMR measurements of the lipid lateral diffusion coefficients in several macroscopically aligned bilayer systems were summarized from previous and new studies. The aim was to carry out a comparison of the translational dynamics for bilayers with various mixtures of l,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), l,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and chicken egg yolk sphingomyelin (eSM), with or without cholesterol. New useful information was obtained on the dynamics in these lipid bilayers that has not been previously appreciated. Thus, we were able to propose that the driving force behind the phase separation into l(d)and l(o)phases evolves from the increasing difficulty to incorpotate DOPC into a highly ordered phase. Our results suggest that DOPC has a preference to be located in a disordered phase, while DPPC and eSM prefer the ordered phase. Quite unexpectedly, CHOL seems to partition into both phases to roughly the same extent, indicating that CHOL has no particular preference for any of the l(d)or l(o) phases, and there are no specific interactions between CHOL and saturated lipids.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Esfingomielinas
/
1,2-Dipalmitoilfosfatidilcolina
/
Colesterol
/
Bicamadas Lipídicas
Idioma:
En
Revista:
Chem Phys Lipids
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Suécia