The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm.
Plant Cell Rep
; 25(10): 1052-8, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-16670900
ABSTRACT
Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g(-1) DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g(-1) DW) and IBS (0.307 mg g(-1) DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Alcaloides de Pirrolizidina
/
Naftoquinonas
/
Raízes de Plantas
/
Lithospermum
Idioma:
En
Revista:
Plant Cell Rep
Assunto da revista:
BOTANICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Polônia