Synthesis of a 3'-naphthamido-LacNAc fluorescein conjugate with high selectivity and affinity for galectin-3.
Carbohydr Res
; 341(10): 1363-9, 2006 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-16697983
ABSTRACT
Described is the synthesis of a fluorescent LacNAc derivative appended with a 3'-deoxy-3'-naphthamido functionality, 2-(fluorescein-5/6-amido)ethyl 3-deoxy-3-(2-naphthamido)-beta-D-galactopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside, which confers high affinity (Kd 170 nM) and selectivity for galectin-3 via a stacking interaction with Arg144. Its use as a selective and sensitive galectin-3 probe is demonstrated with fluorescence polarization measurements.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Galectina 3
/
Dissacarídeos
/
Fluoresceínas
Idioma:
En
Revista:
Carbohydr Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Suécia