Synthesis and evaluation of sequence-specific DNA alkylating agents: effect of alkylation subunits.

Shimizu, Tatsuhiko; Sasaki, Shunta; Minoshima, Masafumi; Shinohara, Ken-ichi; Bando, Toshikazu; Sugiyama, Hiroshi

Shimizu, Tatsuhiko; Sasaki, Shunta; Minoshima, Masafumi; Shinohara, Ken-ichi; Bando, Toshikazu; Sugiyama, Hiroshi.

Afiliação

Shimizu T; Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.

Nucleic Acids Symp Ser (Oxf) ; (50): 155-6, 2006.

Article em En | MEDLINE | ID: mdl-17150864

ABSTRACT

ABSTRACT

We have demonstrated that hairpin pyrrole (Py)- imidazole (Im) polyamide-CBI conjugates selectively alkylate predetermined sequences. In this study, we investigated the effect of alkylation subunits, for example conjugates 1-4 with three types of DNA alkylating units, and Py-Im polyamides with indole linker. Conjugate 3 and 4 selectively alkylated the predetermined sequences as described previously, while conjugates 1 and 2 alkylate at mismatched sites.

Assuntos

Antineoplásicos Alquilantes/química; Antineoplásicos Alquilantes/síntese química; Pareamento Incorreto de Bases; Sequência de Bases; Ciclopropanos/química; DNA/química; Eletroforese em Gel de Poliacrilamida; Imidazóis/química; Indóis/química; Nylons/química; Pirróis/química

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Antineoplásicos Alquilantes Idioma: En Revista: Nucleic Acids Symp Ser (Oxf) Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Japão

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