The efficacy of techniques for the disinfection of artificial sub-surface dentinal caries lesions and their effect on demineralization and remineralization in vitro.
J Dent
; 35(6): 490-5, 2007 Jun.
Article
em En
| MEDLINE
| ID: mdl-17467138
ABSTRACT
OBJECTIVES:
The efficacy of three techniques for the disinfection of artificial sub-surface root caries lesions and their response to subsequent episodes of de- and remineralization was investigated quantitatively in vitro.METHODS:
Sub-surface dentinal lesions (n=20), cut into four experimental blocks and deliberately contaminated with Streptococcus mutans, were subject to either steam autoclaving (121 degrees C, 5min), gamma irradiation (4100Gy), immersion in 0.1% (w/v) thymol-distilled water solution (24h) or reserved as a control. Next, the lesions were incubated aerobically in sterile nutrient broth for 24h at 37 degrees C and resultant cultures plated onto blood agar and neutralisation agar. Ten blocks from each experimental group were then immersed in an acidic buffer solution or exposed to artificial saliva for 5 days. Baseline changes in the mineral content and distribution of the lesions were assessed by transverse microradiography (TMR).RESULTS:
Micro-organisms were recovered from each control block and one block treated by gamma irradiation. Steam autoclaving and immersion in a thymol solution significantly decreased (p<0.05) the amount of mineral lost from the body of lesions subject to a further acid challenge. Mineral ion uptake by lesions exposed to artificial saliva was significantly increased (p<0.05) through disinfection by steam autoclaving.CONCLUSIONS:
Gamma irradiation proved the most acceptable method for the disinfection of sub-surface root dentine lesions having the least adverse effect on demineralization and remineralization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Remineralização Dentária
/
Desinfecção
/
Desmineralização do Dente
/
Cárie Radicular
/
Dentina
Tipo de estudo:
Clinical_trials
Limite:
Humans
Idioma:
En
Revista:
J Dent
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Reino Unido