Electrochemical and spectroscopic characterization of the interaction between DNA and Cu(II)-naringin complex.
J Pharm Biomed Anal
; 45(5): 706-13, 2007 Dec 21.
Article
em En
| MEDLINE
| ID: mdl-17881180
ABSTRACT
In this work the interaction of DNA and the flavonoid-transition metal complex (Cu(II)-naringin complex) is characterized. The interaction was evaluated by using electrochemical ssDNA- and dsDNA-based biosensors and the results were supported by UV, CD and (1)H NMR data. In the electrochemical method, changes in the oxidation peak of the guanine and adenine bases obtained by square wave voltammetry (SWV) showed evidence of the interaction. The variations of the spectroscopic characteristics of DNA and Cu(II)-naringin complex in aqueous medium demonstrated that the predominant interaction mode may be by intercalation. Cu(II)-naringin complex interacts to dsDNA probably via N(7) of guanine site.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
DNA
/
Cobre
/
Flavanonas
/
Eletroquímica
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Brasil