In vitro degradation of endothelin-1 by endopeptidase 24.11 (enkephalinase).
Peptides
; 12(4): 773-8, 1991.
Article
em En
| MEDLINE
| ID: mdl-1788141
ABSTRACT
The breakdown of endothelin-1 by crude membrane preparations of human kidney and choroid plexus was investigated. 125I-labeled endothelin-1 was degraded by both tissues in a phosphoramidon-sensitive way, suggesting a role of endopeptidase 24.11 in the in vitro metabolism of this peptide. Identification of the cleavage sites of purified human renal endopeptidase 24.11 in the sequence of endothelin-1 revealed that bonds involving the amino side of the hydrophobic amino acids (Ser4, Leu6, Val12, Phe14, His16, Leu17, Ile19) were susceptible to cleavage. Endothelin-1 appears thus to be degraded at multiple sites by endopeptidase 24.11 in vitro, producing inactive fragments.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neprilisina
/
Endotelinas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Peptides
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Bélgica